Salvia Biotechnology by Vasil Georgiev & Atanas Pavlov

Author:Vasil Georgiev & Atanas Pavlov
Language: eng
Format: epub
Publisher: Springer International Publishing, Cham

7 Closing Remarks and Future Perspectives

The biotechnological platform established for the two species reviewed in this chapter reveals a wide field of possible developments in Salvia metabolic engineering research. The in vitro grown tissues of S. dolomitica and S. officinalis could be used as perspective model plant systems from Labiatae family in order to produce secondary metabolites or to study the accumulation of VOCs and EO composition in response to chemical and physical elicitors. The recent elucidation of RA metabolic pathway could be used as a base for future projects on evaluation of feasibility to increase metabolite production by Salvia plant tissues, cultivated under controlled environment. The SoHPPR has been cloned and sequenced, opening the way for further genetic engineering procedures. Despite that no clear evidences for overproduction of RA in transformed calli could be provided yet, the effective transformation protocols have been developed. To the best of our knowledge, this is the first transformation protocol applied to Salvia with positive outcomes, which could be used as a good starting point for future research. To date, this is the first report for Salvia officinalis callus transformation, and this protocol is suitable for developing further experiments with cell suspensions with rapid in planta detection of secondary metabolites.

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